System of grading of 20 Mycological characters.


Grades 1 2 3 4 5 6
Color of old slant culture (1) Brown   Brown with green     Green
Diameter (mm) of colony at 34℃ (2) 20-30 30-40 40-50 50-60 60-70 70-80
Colony Reverse side Wrinkling (3) -
plane
+
faint
++ +++ ++++ +++++
evident
Color (4) -
colorless
+
faint
++ +++ ++++ +++++
deep
Sclerotium (5) -
absence
+
few
++ +++ ++++ +++++
abundant
Roughness of stalk (6) -
smooth
+ ++ +++ ++++
coarsely rough
+++++
coarsely rough
Length of stalk(mm) (7) 0.2-0.6 0.6-1.2 0.8-1.4 1.2-1.8 1.6-2.8 3-6
Diameter of vesicle(μ) (8) 6-15 15-25 20-30 25-35 30-40 40-80
Roughness of conidia (9) -
smooth
+ ++ +++ ++++
echinulate to
tuberculate
+++++
echinulate to
tuberculate
Diameter of conidia(μ) (10) 3-4 4-5 5.2-5.6 5.8-6.2 6-7 7-10
Ratio (%) of biseriate sterigmata (11) -
under
several %
+
several %
++
over several %
+++
20%
++++
40%
+++++
over 60%
Production Kojic acid (12)
-
none to negligible
+
trace
*
++
+++
++++
+++++
much
Pigments (13)
Aflatoxins (14)
Amylase (15)
Coloration of hydroquinon (16) -
uncolored
+
faint
++ +++ ++++ +++++
dark red
Reduction of Methylene blue (17) -
over 24hr
+
20hr
++
9hr
+++
6hr
++++
3hr
+++++
under 1hr
Browning of rice koji (18) -
none
+
faint
++ +++ ++++ +++++
black
Color of conidia MCA-medium(19) -
none
+
pale pink
++ +++ ++++ +++++
dark pink
MCH-medium(20)
*sometimes showing some spots on Thin-layer chromatogram and UV-absorption spectra similar to those of aflatoxins, being unstable.

(Murakami H, Classification of the Koji Mold., J. Gen. Appl. Microbiol., 17, 281 (1971))


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